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Analysis of Histone Antibody Specificity with Peptide Microarrays
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Quality of histone modification antibodies undermines chromatin biology research

Goran Kungulovski1, Albert Jeltsch1

  • 1Institute of Biochemistry, Stuttgart University, Stuttgart, 70569, Germany.

F1000Research
|February 5, 2016
PubMed

Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.

Keywords:
antibodieshistone modificationsquality controlrecombinant proteins

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