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Updated: Mar 26, 2026

Basophil Activation Test for Allergy Diagnosis

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Published on: May 31, 2021

Isolation of Human Basophils.

John T Schroeder¹, Anja P Bieneman¹

¹Johns Hopkins University School of Medicine, Baltimore, Maryland.

Current Protocols in Immunology

|February 3, 2016

Summary

This study presents refined laboratory protocols for isolating human basophils, a rare immune cell type. These methods enable researchers to study basophil biology in various in vitro immune responses.

Area of Science:

Immunology
Cell Biology
Hematology

Background:

Basophils are rare circulating leukocytes, typically <1% of the total.
Their low abundance poses significant challenges for in vitro studies.
Understanding basophil function is crucial for immunology research.

Purpose of the Study:

To describe optimized laboratory protocols for isolating human basophils.
To enable researchers to study basophil in vitro biology.
To facilitate investigations into both IgE-dependent and -independent immune responses.

Main Methods:

Refined laboratory protocols developed over 25 years.
Isolation of human basophils from blood.
Preparation of basophils for various in vitro assays.

Keywords:

immune disease immunologic studies in humans in vitro assays for immune cell function innate immunity

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Main Results:

Successfully isolated human basophils.
Developed robust protocols for basophil preparation.
Enabled diverse in vitro analyses of basophil function.

Conclusions:

Optimized protocols overcome challenges in isolating rare basophils.
These methods support advanced research into basophil-mediated immune responses.
Facilitates the study of basophil roles in health and disease.