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Reprogrammable multiplexed detection of circulating oncomiRs using hybridization chain reaction.

Muhit Rana1, Mustafa Balcioglu, Maya Kovach

  • 1Department of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, USA. myigit@albany.edu.

Chemical Communications (Cambridge, England)
|February 4, 2016
PubMed
Summary

This study developed a novel method for detecting breast cancer oncomiRs using hybridization chain reaction (HCR) and gold nanoparticles. The system is reprogrammable for multiplexed detection of specific oncomiRs in liquid biopsies.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Circulating microRNAs (oncomiRs) are key biomarkers for breast cancer detection and prognosis.
  • Multiplexed detection of multiple oncomiRs is crucial for comprehensive disease state analysis.
  • Existing detection methods often require complex instrumentation and lack reprogrammability.

Purpose of the Study:

  • To develop a reprogrammable and multiplexed detection platform for circulating oncomiRs.
  • To couple hybridization chain reaction (HCR) with gold nanoparticles for enhanced sensitivity.
  • To enable visual detection of oncomiRs without analytical instruments.

Main Methods:

  • Utilized the DNA polymerization of HCR and the plasmonic properties of gold nanoparticles.
  • Designed a system where changing the HCR initiator allows reprogramming for different oncomiR targets.
  • Demonstrated detection of miR-10b, miR-21, and miR-141 individually and simultaneously.

Main Results:

  • Achieved visual detection of as little as 20 femtomoles of each oncomiR.
  • Successfully reprogrammed the nanoparticle assembly for various detection combinations by altering HCR initiators.
  • Validated detection in an RNA pool from a liquid biopsy mimic of breast cancer.

Conclusions:

  • Developed a versatile and sensitive platform for oncomiR detection.
  • The reprogrammable nature allows for flexible and simultaneous detection of multiple breast cancer biomarkers.
  • This method offers a promising tool for non-invasive breast cancer diagnostics using liquid biopsies.