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Related Experiment Video

Updated: Mar 26, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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CYTOTOXIC EFFECTS OF METAPHASE-ARRESTING METHODS IN BARLEY.

A Temel, N Gozukirmizi

    Tsitologiia I Genetika
    |February 5, 2016
    PubMed
    Summary

    Amiprophos-methyl (APM) and cell cycle synchronization (CCS) effectively induced metaphase arrest in barley, allowing cell recovery. Colchicine (COL) also arrested metaphase but caused irreversible damage and micronucleus formation.

    Area of Science:

    • Plant genetics and cytology
    • Agricultural science

    Background:

    • Metaphase-arresting agents are crucial for cytogenetic studies.
    • Understanding their effects on plant cell division and growth is essential for optimizing experimental protocols.

    Purpose of the Study:

    • To evaluate the efficacy of amiprophos-methyl (APM), colchicine (COL), and cell cycle synchronization (CCS) using APM and hydroxyurea (HU) on barley (Hordeum vulgare).
    • To assess their impact on growth, metaphase index, and cytogenetic abnormalities.

    Main Methods:

    • Barley seeds were germinated, and seedlings were treated with APM, COL, or a CCS protocol (HU followed by APM).
    • Metaphase indices, nuclear aberrations, and post-treatment growth were analyzed.

    Main Results:

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  • APM and CCS treatments resulted in significant metaphase indices (12.57% and 38.82%, respectively) with subsequent cell recovery.
  • Colchicine induced metaphase arrest (14.10%) but led to nuclear aberrations (11.15%) and irreversible growth inhibition.
  • Colchicine was also effective in inducing micronucleus formation.

    • Conclusions:

    • APM and CCS are effective and reversible metaphase-arresting agents for barley, suitable for cell cycle studies.
    • Colchicine, while effective for metaphase arrest and micronucleus induction, causes detrimental and irreversible effects on barley cell division and growth.