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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR01:59

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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Mar 26, 2026

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
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Efficient Targeted Genome Modification in Maize Using CRISPR/Cas9 System.

Chao Feng1, Jing Yuan2, Rui Wang2

  • 1State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Journal of Genetics and Genomics = Yi Chuan Xue Bao
|February 5, 2016
PubMed
Summary
This summary is machine-generated.

The CRISPR/Cas9 system efficiently modifies maize genomes, creating targeted mutations in both active and inactive DNA regions. This powerful gene-editing tool demonstrates high efficiency and precision in maize, paving the way for crop improvement.

Keywords:
CRISPR/Cas9Heterochromatic regionMaizeTargeted genome modification

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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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Area of Science:

  • Plant Biotechnology
  • Genetics
  • Molecular Biology

Background:

  • The CRISPR/Cas9 system offers precise genome editing capabilities.
  • Its application in maize is crucial for genetic research and crop improvement.

Purpose of the Study:

  • To evaluate the efficiency and specificity of the CRISPR/Cas9 system for targeted genome modification in maize.
  • To investigate its effectiveness in both euchromatic and heterochromatic regions of the maize genome.

Main Methods:

  • Transformation of sgRNA-Cas9 constructs into maize protoplasts.
  • Targeting the Zmzb7 marker gene and analyzing induced indel mutations.
  • Sequencing genomic DNA from transformed protoplasts to assess mutation efficiency and off-target effects, particularly in centromeric and pericentromeric regions.

Main Results:

  • Successful targeted genome modification in maize, evidenced by the identification of an albino mutant seedling.
  • Efficient mutation induction in heterochromatic regions of the maize genome, regardless of gene expression status.
  • Absence of detectable off-target mutations at similar sites lacking a PAM or with multiple mismatches.

Conclusions:

  • The CRISPR/Cas9 system is a robust and efficient tool for genome editing in maize.
  • It effectively targets both euchromatic and heterochromatic regions, offering broad applicability.
  • The system exhibits high specificity, with minimal off-target mutations observed.