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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR

PCR

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Related Experiment Video

Updated: Mar 26, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Quantitative Real-Time PCR: Recent Advances.

Charanjeet Singh¹, Sinchita Roy-Chowdhuri²

¹Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 5, 2016

Summary

This summary is machine-generated.

Quantitative real-time polymerase chain reaction (qPCR) allows simultaneous DNA amplification and quantification in real time. This technique, utilizing fluorescent detection, offers rapid amplification and genotyping for various applications.

Keywords:

Fluorescent genotyping Hybridization probe Intercalation probe Minimal residual disease Molecular beacon Nucleic acid quantification Polymerase chain reaction (PCR)Quantitative PCR Real-time PCR q-PCR

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Last Updated: Mar 26, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

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Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

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Published on: September 24, 2015

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Quantitative real-time polymerase chain reaction (qPCR) is a key molecular biology technique.
It enables simultaneous DNA amplification and quantification within a closed-tube system.
qPCR provides real-time monitoring of the amplification process.

Purpose of the Study:

To describe the properties and uses of fluorescent detection systems in qPCR.
To highlight the utility of qPCR for DNA quantification.
To discuss applications in amplification and genotyping.

Main Methods:

Utilizes fluorescent detection systems for real-time monitoring.
Employs melting curve analysis for product characterization.
Applies closed-tube system for simultaneous amplification and quantification.

Main Results:

qPCR allows for absolute or relative quantification of target DNA.
The technique provides rapid amplification and genotyping.
Various fluorescent detection systems are suitable for qPCR quantification.

Conclusions:

Fluorescent detection systems are integral to qPCR.
qPCR is a versatile tool for DNA quantification and analysis.
The described systems enhance the efficiency and speed of molecular assays.