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Updated: Mar 26, 2026

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells
Published on: July 5, 2024
Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
Svend Roesen Madsen1, Hussam Hassan Nour-Eldin1, Barbara Ann Halkier2
1DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark.
Abstract:
The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.
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