Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescent staphylococci as microbeads.

G Szabó1, S Damjanovich

  • 1Department of Biophysics, University Medical School of Debrecen, Hungary.

Cytometry
|November 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD4 changes conformation upon ligand binding.

Journal of immunology (Baltimore, Md. : 1950)·1992
Same author

Induction of transforming growth factor-beta and prostaglandin E2 production by ethanol in human monocytes.

Journal of leukocyte biology·1992
Same author

A voltage-gated calcium channel is linked to the antigen receptor in Jurkat T lymphocytes.

FEBS letters·1992
Same author

Opposite actions of oxytocin and vasopressin in the development of cocaine-induced behavioral sensitization in mice.

Pharmacology, biochemistry, and behavior·1992
Same author

Effects of cocaine on the contents of neurohypophyseal hormones in the plasma and in different brain structures in rats.

Neuropeptides·1992
Same author

Oxytocin blocks the development of heroin-enkephalin cross-tolerance in mice.

Pharmacology, biochemistry, and behavior·1992
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

This study used fluorescein isothiocyanate-labeled Staphylococcus bacteria to detect cell-surface antigens via indirect immunofluorescence. This method allows for the visualization and quantification of antigen expression on cells.

Area of Science:

  • Microbiology
  • Immunology
  • Biotechnology

Background:

  • Protein A from Staphylococcus aureus Cowan I is a well-characterized surface protein.
  • Indirect immunofluorescence assays (IIFA) are crucial for detecting specific antigens.
  • Fluorescent labeling enables sensitive detection and quantification in biological samples.

Purpose of the Study:

  • To develop and validate a method for detecting specific cell-surface antigen expression.
  • To utilize fluorescein isothiocyanate (FITC) labeled bacteria as a reagent in immunofluorescence.

Main Methods:

  • Staphylococcus aureus Cowan I bacteria were fixed and labeled with fluorescein isothiocyanate (FITC).
  • These labeled bacteria served as a secondary reagent in an indirect immunofluorescence assay.

Related Experiment Videos

  • Cell-surface antigen expression was analyzed using fluorescence microscopy and flow cytometry.

    • Main Results:

    • The labeled bacteria successfully acted as a secondary reagent in the indirect immunofluorescence assay.
    • Fluorescence microscopy provided visual confirmation of antigen localization.
    • Flow cytometry enabled quantitative analysis of cell-surface antigen expression.

    Conclusions:

    • FITC-labeled Staphylococcus aureus Cowan I provides an effective secondary reagent for indirect immunofluorescence.
    • This technique is suitable for studying specific cell-surface antigen expression.
    • The combination of fluorescence microscopy and flow cytometry offers robust detection and quantification.