Related Experiment Video
Updated: Mar 26, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
Expression profile of long non-coding RNAs in colorectal cancer: A microarray analysis
Jia Luo1, Luning Xu2, Yigui Jiang1
1Department of Gastroenterology, The Sanming First Hospital Affiliated to Fujian Medical University, Sanming, Fujian 365000, P.R. China.
Long non-coding RNAs (lncRNAs) are implicated in colorectal cancer (CRC) pathogenesis. This study identified differentially expressed lncRNAs and mRNAs in CRC tissues, revealing their correlation with key cancer pathways like Wnt signaling.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Colorectal cancer (CRC) is a leading cause of cancer mortality worldwide.
- Understanding CRC pathogenesis and identifying novel therapeutic targets are critical.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their role in tumor development.
Purpose of the Study:
- To investigate the expression profiles of lncRNAs and mRNAs in colon adenocarcinoma.
- To identify differentially expressed genes and associated pathways in CRC.
- To explore the correlation between lncRNAs and CRC-related signaling pathways.
Main Methods:
- Microarray analysis of lncRNA and mRNA expression in 4 colon adenocarcinoma tissues and paired adjacent normal tissues.
- Bioinformatic analysis including differential expression analysis, pathway enrichment, and gene ontology analysis.
- Coding-non-coding gene co-expression analysis to identify functional correlations.
Main Results:
- A total of 3,523 lncRNAs and 2,515 mRNAs were found to be differentially expressed in CRC tissues.
- Upregulated transcripts were enriched in DNA metabolic processes, while downregulated transcripts were enriched in organic hydroxyl compound metabolic processes.
- Differentially expressed lncRNAs showed a strong correlation with components of the Wnt signaling pathway, crucial in CRC.
Conclusions:
- lncRNA dysregulation is significantly involved in the development of colorectal cancer.
- These findings suggest lncRNAs may serve as novel diagnostic markers for CRC.
- lncRNAs could represent potential therapeutic targets for colorectal cancer treatment.
More Related Videos
10:33Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
Published on: April 29, 2014
08:12Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
Related Concept Videos
lncRNA - Long Non-coding RNAs
lncRNA - Long Non-coding RNAs
Non-LTR Retrotransposons
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
MicroRNAs