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Potential Dual Role of Activating Transcription Factor 3 in Colorectal Cancer

Xiaojing Jiang1, Kui-Jin Kim1, Taekyu Ha1

  • 1Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD, U.S.A.

Anticancer Research
|February 7, 2016
PubMed
Abstract

Insights

Activating transcription factor 3 (ATF3) impacts colorectal cancer cell phenotypes. ATF3 influences apoptosis, cell migration, and epithelial mesenchymal transition (EMT), suggesting a dual role in cancer progression.

Area of Science:

  • Molecular biology
  • Cancer research
  • Genetics

Background:

  • Activating transcription factor 3 (ATF3) is part of the ATF/CREB transcription factor family.
  • ATF3 is a potential molecular target for cancer therapy.

Purpose of the Study:

  • Investigate the influence of ATF3 on cancer-related phenotypes in colorectal cancer.
  • Determine ATF3's role in apoptosis, cell cycle, cell migration, and epithelial mesenchymal transition (EMT).

Main Methods:

  • Overexpression of ATF3 in human colorectal cancer cells.
  • Assessed effects on apoptosis, cell cycle, migration, and EMT.
  • Utilized luciferase assay to evaluate Bcl-2 promoter activity.

Main Results:

  • ATF3 downregulated B-cell lymphoma-2 (Bcl-2) expression and promoter activity.
  • ATF3 enhanced collective cell migration and cluster of differentiation 44 (CD44) expression.
  • ATF3 decreased retinoblastoma (Rb) expression, EMT-inducing transcription factors, and β-catenin.

Conclusions:

  • ATF3 exhibits a dichotomous role in colorectal cancer.
  • ATF3 influences both apoptosis and metastasis in human colorectal cancer cells.
Keywords:
Activating transcription factor 3Bcl-2apoptosiscolorectal cancermigration

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