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Updated: Mar 26, 2026
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Published on: August 17, 2019
A target responsive aptamer machine for label-free and sensitive non-enzymatic recycling amplification detection of
Xin Li1, Ying Peng, Yaqin Chai
1Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China. yunatswu@swu.edu.cn.
Abstract:
Based on a new target-triggered aptamer molecular machine, a label-free and non-enzymatic target recycling amplification strategy for sensitive fluorescence detection of ATP in human serums is described. The presence of the target ATP together with the DNA fuel strand initiates the operation of the aptamer machine and leads to cyclic reuse of ATP and the release of many G-quadruplex sequences, which associate with a fluorescent dye to generate significantly amplified fluorescence signals to achieve sensitive detection of ATP.
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