Related Experiment Videos
Diabetic syndrome induced by monosodium aspartate administration in Microtus arvalis Pallas
Abstract:
Administration of monosodium aspartate (MSA) to neonatal voles, Microtus arvalis Pallas induced neuronal necrosis in several brain regions including the arcuate and ventromedial hypothalamic nuclei. In the growing stage, 56.7% showed glycosuria. In the mild diabetic voles, blood glucose concentrations rose to 180 mg/dl (72 mg/dl in normal), and plasma insulin levels were also increased to 112 microU/ml (21 microU/ml in normal). In the severe diabetic voles, on the other hand, blood glucose concentrations rose over 250 mg/dl, and plasma insulin levels decreased under 10 microU/ml. Histopathological examination of the pancreatic islets revealed moderately enlarged islets, and nuclear hypertrophy and partial degranulation of B cells in the mild diabetic voles, and moderately to markedly enlarged islets, and marked degranulation, vacuolation and accumulation of glycogen granules of B cells in the severe diabetic voles. These results indicated that the diabetic syndrome in herbivorous voles was induced by administration of MSA and diabetic voles fell into marked insulin deficiency in the severe stage.
Insights
Monosodium aspartate (MSA) administration to neonatal voles induced diabetes, causing neuronal damage and impaired insulin function. Severe cases showed significant insulin deficiency, highlighting MSA
Area of Science:
- Neuroscience
- Endocrinology
- Toxicology
Background:
- Monosodium aspartate (MSA) is an excitotoxin that can affect neuronal development.
- Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and impaired insulin secretion or function.
Purpose of the Study:
- To investigate the effects of MSA administration on the development of diabetes in neonatal voles.
- To characterize the neuropathological and metabolic changes associated with MSA-induced diabetes.
Main Methods:
- Neonatal voles were administered MSA.
- Blood glucose and plasma insulin levels were measured.
- Glycosuria was assessed.
- Pancreatic islets were examined histopathologically.
Main Results:
- MSA administration induced neuronal necrosis in hypothalamic nuclei.
- 56.7% of voles developed glycosuria.
- Mildly diabetic voles showed elevated blood glucose and insulin levels.
- Severely diabetic voles exhibited hyperglycemia and significantly decreased insulin levels.
- Histopathology revealed B cell changes in pancreatic islets, including hypertrophy, degranulation, vacuolation, and glycogen accumulation.
Conclusions:
- MSA induces a diabetic syndrome in herbivorous voles.
- The diabetic syndrome is characterized by neuronal damage and progressive B cell dysfunction.
- Severe diabetes in voles is associated with marked insulin deficiency.