Related Experiment Video
Updated: Mar 26, 2026

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Published on: July 6, 2012
Translation regulation via nascent polypeptide-mediated ribosome stalling
Daniel N Wilson1, Stefan Arenz2, Roland Beckmann1
1Gene Center and Department of Biochemistry, Feodor-Lynenstr. 25, 81377 Munich, Germany; Center for Integrated Protein Science, Munich (CiPSM), Feodor-Lynenstr. 25, 81377 Munich, Germany.
Abstract:
As the nascent polypeptide chain is being synthesized, it passes through a tunnel within the large ribosomal subunit. Interaction between the nascent polypeptide chain and the ribosomal tunnel can modulate the translation rate and induce translational stalling to regulate gene expression. In this article, we highlight recent structural insights into how the nascent polypeptide chain, either alone or in cooperation with co-factors, can interact with components of the ribosomal tunnel to regulate translation via inactivating the peptidyltransferase center of the ribosome and inducing ribosome stalling.
Related Concept Videos
Termination of Translation
Translational Regulation
Improving Translational Accuracy
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay
Cotranslational Protein Translocation
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...

