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An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
Published on: May 22, 2020
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In-electrode vs. on-electrode: ultrasensitive Faraday cage-type electrochemiluminescence immunoassay
Zhiyong Guo1, Yuhong Sha1, Yufang Hu1
1School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. guozhiyong@nbu.edu.cn.
Abstract:
A new-concept of an "in-electrode" Faraday cage-type electrochemiluminescence immunoassay (ECLIA) method for the ultrasensitive detection of neurotensin (NT) was reported with capture antibody (Ab1)-nanoFe3O4@graphene (GO) and detector antibody (Ab2)&N-(4-aminobutyl)-N-ethylisoluminol (ABEI)@GO, which led to about 1000-fold improvement in sensitivity by extending the Helmholtz plane (OHP) of the proposed electrode assembly effectively.

