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Use of Human Perivascular Stem Cells for Bone Regeneration
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Data on bone marrow stem cells delivery using porous polymer scaffold.

Ramasatyaveni Geesala1, Nimai Bar2, Neha R Dhoke1

  • 1Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500007, India; Academy of Scientific & Innovative Research (AcSIR), 2 Rafi Marg, New Delhi 110001, India.

Data in Brief
|February 11, 2016
PubMed
Summary

A novel porous polymer scaffold effectively delivers bone marrow stem cells (BMSCs) to wound sites, enhancing tissue repair. This biocompatible vehicle improves stem cell survival and integration for regenerative medicine applications.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Transplanted stem cells often have low bioavailability and survival rates at injury sites.
  • Effective delivery vehicles are crucial for enhancing stem cell efficacy in regenerative therapies.
  • Existing methods require improvement for on-site stem cell delivery and survival.

Purpose of the Study:

  • To evaluate a porous, biocompatible, and biodegradable polymer network for delivering bone marrow stem cells (BMSCs) to a murine wound model.
  • To characterize BMSCs and analyze their penetration and engraftment within the polymer scaffold.
  • To investigate the mechanism of stem cell delivery and compare different transplantation routes.

Main Methods:

  • Characterization of BMSCs for pluripotency and surface markers.
  • Image analysis of cellular penetration into the PEG-PU polymer network.
  • Murine excisional splint wound model with bone marrow transplantation chimeras.
  • Enzymatic activation analysis involving MMP-2 and MMP-13.

Main Results:

  • Successful delivery of BMSCs to the wound site using the porous polymer scaffold.
  • Demonstrated cellular penetration into the PEG-PU network, facilitated by MMP-2 and MMP-13.
  • Comparative analysis of transplantation routes revealed varying degrees of BMSC engraftment.
  • Confirmed engraftment of specific BMSC populations (Sca-1(+)Lin(-)CD133(+)CD90.2(+)) by post-surgery day 10.

Conclusions:

  • The porous polymer network serves as an effective scaffold for on-site delivery of BMSCs.
  • The scaffold protects transplanted BMSCs and promotes their engraftment and survival at the wound site.
  • This delivery system holds promise for enhancing wound tissue repair and regenerative medicine strategies.