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Updated: Mar 26, 2026
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh
Vivian Huerta1, Yassel Ramos1, Alexis Yero1
1Center for Genetic Engineering and Biotechnology (CIGB), Cuba.
Abstract:
The four serotypes of dengue virus (DENV1-4) are the causal agents of the emerging disease Dengue Fever and its severe forms. DENV is inoculated into human blood through a mosquito bite. Thus, plasma is an important media for DENV dissemination in infected persons and several important interactions should take place for the virus with human plasma proteins that strongly influence or may determine the course of the infection. This dataset contains 239 proteins identified in the elution fractions of human plasma subjected to DE-52 anion exchange chromatography. Data on DENV2 infection of Huh 7.5 cells in presence of the human plasma fraction is also presented.
Insights
This study identifies 239 human plasma proteins interacting with dengue virus (DENV). Understanding these interactions is key to controlling Dengue Fever, an emerging infectious disease transmitted by mosquitoes.
Area of Science:
- Virology
- Immunology
- Proteomics
Background:
- Dengue virus (DENV) causes Dengue Fever and severe disease.
- Mosquito bites transmit DENV to humans, making plasma a key medium for viral spread.
- Interactions between DENV and human plasma proteins significantly influence infection outcomes.
Purpose of the Study:
- To identify human plasma proteins that interact with dengue virus.
- To provide a dataset of proteins found in human plasma fractions after chromatography.
- To investigate the effect of human plasma fractions on DENV2 infection of cells.
Main Methods:
- Human plasma was subjected to DE-52 anion exchange chromatography.
- Proteins in elution fractions were identified using mass spectrometry.
- DENV2 infection of Huh 7.5 cells was studied in the presence of plasma fractions.
Main Results:
- A dataset of 239 proteins identified in human plasma elution fractions was generated.
- Data on the impact of these plasma fractions on DENV2 cell infection is presented.
Conclusions:
- The study provides a valuable resource of potential DENV-interacting plasma proteins.
- Further research can explore the functional roles of these identified proteins in DENV pathogenesis and host response.
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