Acrylic Resin Molding Based Head Fixation Technique in Rodents

Area of Science:

• Neuroscience
• Animal Research Methodology

Background:

• Traditional head fixation methods often involve complex, surgically attached metallic frames.
• Fabricating and implementing these metallic frames can be challenging in standard laboratory environments.

Purpose of the Study:

• To develop and present a novel, easily implementable head fixation technique for laboratory animals.
• To offer an alternative to traditional metallic head fixation systems, particularly for facilities with limited fabrication capabilities.

Main Methods:

• A custom head fixation bar and a molded head mounter were designed.
• The head mounter was constructed during implantation surgery using dental acrylic resin, integrated with skull implants.
• The head mounter was designed to interface with the fixation bar for secure head immobilization.

Main Results:

• The novel method allows for convenient and rigid head fixation by inserting the molded head mounter into the fixation bar.
• The technique is compatible with standard implantation surgeries involving acrylic resins.
• Successfully demonstrated a practical alternative to CNC-machined metal head-posts.

Conclusions:

• The developed head fixation method is practical, cost-effective, and easily applicable in diverse laboratory settings.
• This technique enhances accessibility for researchers needing reliable animal head immobilization without specialized equipment.
• The study provides a valuable alternative for neuroscience and behavioral research requiring stable head fixation.