Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Complex Assembly

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A spike-binding protein as a versatile tool to detect and inhibit transmissible gastroenteritis virus.

Virology·2026

Same author

PEG/Folic Acid Coloaded Er:Y<sub>2</sub>O<sub>3</sub> Upconversion Nanoparticles: Enhanced Internalization and Potential for High-Contrast Bioimaging in HCT-116 Cells.

ACS omega·2026

Same author

Monomeric Structure of Influenza A Virus NEP/NS2 Obtained With an Artificial Protein Highlights Conformational Plasticity.

Journal of molecular biology·2025

Same author

Inhibition of influenza virus replication by artificial proteins (αReps) targeting its RNA-polymerase.

Antiviral research·2025

Same author

Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis.

Journal of extracellular biology·2025

Same author

Infrared thermochromic antenna composite for self-adaptive thermoregulation.

Nature communications·2024

Same journal

Silicon-Mediated Laser Shock Synthesis of Nanocrystalline Diamonds from Low-Rank Coal.

ACS nano·2026

Same journal

Precursor-Engineered Strategy for Constructing Supported Tetra-Atom Pt Clusters to Boost Propane Dehydrogenation under Direct Resistive Heating.

ACS nano·2026

Same journal

Enterohepatic Circulation of Polystyrene Nanoplastics Promotes Intestinal Inflammation by Impairing Enteric Neurons.

ACS nano·2026

Same journal

Triboelectric Spectroscopy for Identification of Metal Ion Valence States in Aqueous Solutions.

ACS nano·2026

Same journal

Beyond the Continuum Theory: Conductance Scaling and Correlated Imaging in Atom-Scale Artificial Ion Channels.

ACS nano·2026

Same journal

Selenium-Induced Directional Growth of Ultrathin Nanowires with Subnano Amorphous Shells for High-Performance Multifunctional Electrocatalysis.

ACS nano·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 26, 2026

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

Nanoparticles Self-Assembly Driven by High Affinity Repeat Protein Pairing.

Kargal L Gurunatha¹, Agathe C Fournier¹, Agathe Urvoas²

¹Groupe NanoSciences-CEMES, CNRS UPR 8011 , 29 rue J. Marvig, B.P. 94347, F-31055 Toulouse, France.

|February 11, 2016

Summary

This summary is machine-generated.

Researchers designed artificial proteins to direct the self-assembly of gold nanoparticles into thin films. These protein-directed nanoparticle assemblies can be reversibly disassembled, paving the way for new nanomaterials.

Keywords:

dissociation constant gold nanoparticles protein pair reversible self-assembly α-repeat proteins

More Related Videos

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Related Experiment Videos

Last Updated: Mar 26, 2026

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Area of Science:

Biochemistry
Materials Science
Nanotechnology

Background:

Proteins are versatile biological molecules with self-assembly capabilities.
Designing proteins for specific recognition and nanoparticle assembly is an emerging field.
Exploiting protein chemistry for inorganic nanoparticle self-assembly remains largely unexplored.

Purpose of the Study:

To engineer artificial proteins for directed self-assembly of inorganic nanoparticles.
To investigate the protein-directed assembly of gold nanoparticles into functional structures.
To explore the reversibility of protein-nanoparticle aggregates.

Main Methods:

Designed and selected artificial repeat proteins (αRep) with high mutual affinity.
Conjugated αRep proteins onto gold nanoparticles.
Controlled protein density on nanoparticles to influence assembly extent.
Investigated the disassembly of nanoparticle aggregates using free proteins.

Main Results:

Achieved massive colloidal assembly of gold nanoparticles into one-particle thick films.
Demonstrated that lower protein density limits assembly to oligomeric clusters.
Showcased reversible disassembly of nanoparticle aggregates by excess free protein.

Conclusions:

Purpose-designed artificial proteins can effectively direct the self-assembly of gold nanoparticles.
The extent of self-assembly is tunable by controlling protein density.
Protein-directed nanoparticle assemblies offer potential for reversible functional nanomaterials and biosensing applications.