Lactoferrin Levels in Tears are Increased by the Topical Application of Diadenosine Tetraphosphate
Patricia Loma1, Ana Guzman-Aranguez1, Maria J Perez de Lara1
1a Departamento de Bioquímica y Biología Molecular , E.U. Óptica, Universidad Complutense de Madrid , Madrid , Spain.
Purpose:
This study was undertaken to determine the effect of the topical application of diadenosine tetraphosphate on lactoferrin levels in rabbit tears.
Methods:
Diadenosine tetraphosphate was topically instilled in a single-dose, tear samples were collected by micropipette and lactoferrin was measured by Enzyme-Linked ImmunoSorbent Assay (ELISA).
Results:
The concentration of lactoferrin in rabbit tears was significantly increased 1 h after diadenosine tetraphosphate application, remaining elevated for 3 h more. This effect was blocked by P2 receptors antagonists.
Conclusions:
Topical application of diadenosine tetraphosphate stimulates the secretion of lactoferrin in rabbit tears through P2 receptor activation.
