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Updated: Mar 26, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Aptamers in analytics.

Muslum Ilgu¹, Marit Nilsen-Hamilton²

¹Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames IA 50011, USA. ilgu@iastate.edu and Aptalogic Inc., Ames IA 50014, USA.

|February 12, 2016

Summary

This summary is machine-generated.

Nucleic acid aptamers offer a promising, cost-effective alternative to antibodies for analytical applications. These molecules exhibit high specificity and stability, enabling advanced sensor development.

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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

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Published on: November 1, 2019

Area of Science:

Analytical Chemistry
Biotechnology
Molecular Biology

Background:

Nucleic acid aptamers are emerging as superior alternatives to antibodies in analytical science.
They are synthesized via SELEX (Systematic Evolution of Ligands by Exponential Enrichment) for specific target recognition.
Aptamers offer advantages such as small size, low cost, stability, and reusability.

Purpose of the Study:

To review the selection and engineering of aptamers for analytical applications.
To discuss challenges in aptamer development for analytics.
To highlight successful aptamer-based sensor applications.

Main Methods:

Discussion of aptamer selection through iterative SELEX.
Explanation of aptamer engineering for enhanced performance.
Review of various aptamer-based analytical platforms (electrochemical, optical, etc.).

Main Results:

Aptamers demonstrate high specificity and affinity for diverse targets.
Conformational changes upon binding enable novel sensing strategies.
Numerous successful aptamer sensor applications have been reported across different platforms.

Conclusions:

Aptamers are versatile tools for developing sensitive and specific analytical sensors.
Despite advantages, clinical translation and commercialization of aptamer-based devices remain limited.
Further research and development are needed to overcome challenges and broaden aptamer applications in analytics.