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Structure and function of outer dynein arm intermediate and light chain complex.

Toshiyuki Oda1, Tatsuki Abe2, Haruaki Yanagisawa2

  • 1Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan Department of Anatomy and Structural Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan toda@yamanashi.ac.jp.

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Area of Science:

  • Cell Biology
  • Structural Biology
  • Biochemistry

Background:

  • The outer dynein arm (ODA) powers cilia and flagella beating.
  • The 3D structures of ODA's intermediate (ICs) and light chains (LCs) remain largely uncharacterized.

Purpose of the Study:

  • To determine the 3D positions of ODA's ICs and LCs.
  • To investigate the role of ICs and LCs in ODA function and mechanosignaling.

Main Methods:

  • Cryo-electron tomography and structural labeling were used to map ICs and LCs.
  • A chemically induced dimerization system probed OID linker interactions.

Main Results:

  • ICs and LCs localize to the ODA-Beak complex at the OID linker root.
  • The IC2 coiled-coil domain extends from the ODA-Beak to the ODA surface.
  • Cross-linking the OID linker significantly impairs flagellar motility.

Conclusions:

  • The ODA-Beak complex, formed by ICs and LCs, is crucial for ODA structure.
  • The ODA-Beak may mediate mechanosignaling from the OID linker to heavy chains, impacting motility.