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Updated: Mar 25, 2026
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
Published on: October 24, 2018
Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR
Margaux Kaster1,2,3, Sascha Laubinger4,5,6
1Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany.
Abstract:
Nucleosome occupancy in promoter and genic regions can severely influence the transcription levels. Few methods have been established to investigate the nucleosome occupancy along the DNA. In this chapter we describe a detailed protocol to analyze the nucleosome occupancy at a specific locus using MNase-pPCR.
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