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Long non-coding antisense RNA KRT7-AS is activated in gastric cancers and supports cancer cell progression by

B Huang1,2, J H Song2, Y Cheng2

  • 1Department of Gastroenterology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Oncogene
|February 16, 2016
PubMed

Insights

Long non-coding RNA KRT7-AS promotes gastric cancer (GC) by increasing keratin 7 expression. This antisense RNA interacts with KRT7, enhancing its protein levels and driving cancer cell growth and migration.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly implicated in human cancer development.
  • Antisense lncRNAs (ASs) are a class of lncRNAs found in cancers, but their roles are not well understood.

Purpose of the Study:

  • To identify ASs involved in human gastric cancer (GC).
  • To elucidate the mechanisms by which ASs contribute to GC carcinogenesis.

Main Methods:

  • Massively parallel RNA sequencing was performed on GC tissues and cell lines, alongside matched normal samples.
  • Identification and functional analysis of a specific AS, Homo sapiens keratin 7 (KRT7-AS).
  • Assessment of KRT7-AS interaction with KRT7 mRNA and its impact on protein levels, cell proliferation, and migration.

Main Results:

  • KRT7-AS was significantly upregulated in GC tissues and cell lines, with concordant expression to its cognate KRT7.
  • KRT7-AS formed an RNA-RNA hybrid with KRT7, regulating its expression at mRNA and post-transcriptional levels.
  • Overexpression of KRT7-AS, particularly its KRT7-overlapping region, increased keratin 7 protein levels and promoted GC cell proliferation and migration.

Conclusions:

  • lncRNA KRT7-AS acts as a promoter of gastric cancer.
  • KRT7-AS promotes GC, at least partly, by enhancing KRT7 expression.

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