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Updated: Mar 25, 2026

Molecular Entanglement and Electrospinnability of Biopolymers
Published on: September 3, 2014
Activation energy associated with the electromigration of oligosaccharides through viscosity modifier and polymeric
Márta Kerékgyártó1, Gábor Járvás2, Levente Novák3
1Horváth Csaba Laboratory of Bioseparation Sciences, Regional Centre for Molecular Medicine, University of Debrecen, Debrecen, Hungary.
Abstract:
The activation energy related to the electromigration of oligosaccharides can be determined from their measured electrophoretic mobilities at different temperatures. The effects of a viscosity modifier (ethylene glycol) and a polymeric additive (linear polyacrylamide) on the electrophoretic mobility of linear sugar oligomers with α1-4 linked glucose units (maltooligosaccharides) were studied in CE using the activation energy concept. The electrophoretic separations of 8-aminopyrene-1,3,6-trisulfonate-labeled maltooligosaccharides were monitored by LIF detection in the temperature range of 20-50°C, using either 0-60% ethylene glycol (viscosity modifier) or 0-3% linear polyacrylamide (polymeric additive) containing BGEs. Activation energy curves were constructed based on the slopes of the Arrhenius plots. With the use of linear polyacrylamide additive, solute size-dependent activation energy variations were found for the maltooligosaccharides with polymerization degrees below and above maltoheptaose (DP 7), probably due to molecular conformation changes and possible matrix interaction effects.
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