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Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin
Kajal Ghosal1,2, Anton Manakhov3, Lenka Zajíčková3
1Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, 686560, India. kajal.ghosal@gmail.com.
AAPS Pharmscitech
|February 18, 2016
Summary
Biodegradable nanofibers made from poly(ε-caprolactone) (PCL), with collagen coating or titanium dioxide incorporation, show promise as scaffolds for skin tissue engineering and wound healing applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Biodegradable polymers like poly(ε-caprolactone) (PCL) are crucial for tissue engineering scaffolds.
- Surface modification and incorporation of nanoparticles can enhance scaffold properties for specific applications.
- Developing compatible scaffolds is essential for successful skin tissue regeneration.
Purpose of the Study:
- To prepare and characterize poly(ε-caprolactone) (PCL) nanofibers, collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL nanofibers (TiO2-i-PCL).
- To evaluate the surface and structural compatibility of these nanofiber scaffolds for skin tissue engineering.
- To assess the biocompatibility and potential of these composite scaffolds for skin wound healing.
Main Methods:
- Electrospinning technique was employed to fabricate PCL, Col-c-PCL, and TiO2-i-PCL nanofibers.
- Scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurements, energy-dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS) were used for characterization.
- MTT assay and cell adhesion studies with L929 fibroblast cell line were conducted to evaluate biocompatibility.
Main Results:
- SEM confirmed the formation of bead-free nanofibers.
- FTIR verified the presence of collagen on the PCL scaffold.
- Contact angle measurements indicated increased hydrophilicity for Col-c-PCL and TiO2-i-PCL.
- EDX and XPS revealed the molecular-level distribution of TiO2.
- MTT assay and cell adhesion studies demonstrated good cell viability and fibroblast attachment.
Conclusions:
- The prepared PCL-based composite nanofibers exhibit suitable surface and structural properties for skin tissue engineering.
- Collagen coating and TiO2 incorporation enhance scaffold hydrophilicity and material distribution.
- These composite nanofibers support fibroblast viability and attachment, indicating potential as tissue-engineered constructs for skin wound healing.
