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Related Experiment Video

Updated: Mar 25, 2026

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
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How Tissue Mechanical Properties Affect Enteric Neural Crest Cell Migration.

N R Chevalier1, E Gazguez2, L Bidault3,4,5

  • 1Laboratoire Matière et Systèmes Complexes, Université Paris-Diderot/CNRS UMR 7057, 10 rue Alice Domon et Léonie Duquet, 75013 Paris, France.

Scientific Reports
|February 19, 2016
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Summary
This summary is machine-generated.

Neural crest cells (ENCCs) migrate best on soft substrates and actively degrade collagen. Gut tissue stiffens during development, influencing ENCC colonization and potentially causing diseases like Hirschsprung disease.

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Area of Science:

  • Developmental biology
  • Cell biology
  • Biophysics

Background:

  • Neural crest cells (NCCs) are crucial for vertebrate development.
  • Defects in NCC migration cause congenital diseases, including Hirschsprung disease.
  • Enteric NCCs (ENCCs) colonize the gut during embryonic development.

Purpose of the Study:

  • Investigate how environmental stiffness and structure affect ENCC migration.
  • Understand the mechanical cues guiding ENCCs in the developing gastrointestinal tract.

Main Methods:

  • Tensile stretching and atomic force microscopy (AFM) to measure tissue stiffness.
  • Second-harmonic generation (SHG) microscopy to visualize collagen organization.
  • Ex-vivo 2D migration assays and 3D collagen gel experiments.
  • Metalloprotease inhibition to assess collagen degradation.

Main Results:

  • Gut mesenchyme softens initially, then stiffens during ENCC colonization.
  • Collagen fibers organize and enrich as the gut stiffens.
  • ENCCs prefer soft substrates for migration.
  • ENCC migration speed decreases with increasing matrix stiffness in 3D gels.
  • ENCCs actively degrade collagen to facilitate migration.

Conclusions:

  • Tissue mechanical properties, particularly stiffness and collagen organization, significantly influence ENCC migration.
  • ENCCs' ability to degrade collagen is essential for their progression.
  • Understanding these mechanical interactions is key to deciphering developmental processes and diseases like Hirschsprung disease.