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Multiscale Imaging to Monitor Functional SHED-Supported Engineered Vessels.

E Chatzopoulou1,2, N Bousaidi1, T Guilbert3

  • 1Université Paris Cité, URP2496 Pathologies, Imagerie et Biothérapies Orofaciales et Plateforme Imagerie du Vivant, Montrouge, France.

Journal of Dental Research
|September 18, 2024
PubMed
Summary
This summary is machine-generated.

Stem cells from human exfoliating deciduous teeth (SHED) combined with endothelial cells in 3D-printed scaffolds promote functional blood vessel growth for orofacial tissue engineering. This approach enhances vascularization and reduces leakage in engineered grafts.

Keywords:
angiogenesiscollagenmagnetic resonance imagingnuclear medicinestem cellstissue engineering

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Vascular Biology

Background:

  • Orofacial tissue regeneration is limited by poor vascular supply.
  • Prevascularized engineered constructs offer a solution for rapid graft vascularization.
  • Dental pulp stem cells possess angiogenic properties beneficial for tissue engineering.

Purpose of the Study:

  • To establish a preclinical model of prevascularized constructs using stem cells from human exfoliating deciduous teeth (SHED).
  • To functionally analyze in vivo angiogenesis, vascular perfusion, and permeability of these constructs.
  • To evaluate the potential of SHED in promoting functional vascular networks for orofacial tissue engineering.

Main Methods:

  • Three cell-loaded hydrogel constructs (SHED-HUVEC, HUVEC with SHED-conditioned medium, SHED alone) were fabricated in polylactic acid (PLA) grids.
  • Constructs were ectopically implanted in athymic mice.
  • In vivo PETscan, ex vivo micro-CT, 2-photon microscopy with lectin/dextran injection were used for analysis.

Main Results:

  • The SHED-HUVEC group showed significantly increased radiotracer uptake (angiogenesis) on PETscan at day 10.
  • Micro-CT confirmed greater vascular volume in SHED-HUVEC constructs at day 30.
  • SHED-HUVEC constructs exhibited enhanced blood perfusion and reduced vascular leakage, indicating functional vessel maturation.

Conclusions:

  • Novel PLA-based prevascularized constructs loaded with SHED and HUVECs promote functional human-engineered vessels.
  • SHED actively contribute to the maturation and stability of engineered microvascular networks.
  • PETscan is validated as a tool for early detection of in vivo angiogenesis in tissue engineering grafts.