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A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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A competitive and reversible deactivation approach to catalysis-based quantitative assays.

Kazunori Koide1, Matthew P Tracey1, Xiaodong Bu2

  • 1Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, USA.

Nature Communications
|February 20, 2016
PubMed
Summary

This study introduces a novel method for optical assays that prevents signal saturation and broadens the dynamic range. By enabling reactions to autonomously stall and restart, it allows for accurate measurements across five orders of magnitude.

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