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Updated: Mar 25, 2026

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Published on: October 15, 2014
Identification and antimicrobial susceptibility testing of positive blood culture isolates from briefly incubated
Mónica Ballestero-Téllez1, Esther Recacha1, Marina de Cueto2
1Unidad Intercentros de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Virgen Macarena-Virgen del Rocío, Sevilla, España.
Introduction:
Mass spectrometry Matrix-Assisted Laser Desorption-Ionization Time-of-Flight (MALDI-TOF) helps in the rapid identification of microorganisms causing blood stream infection. Rapid and reliable methods are required to decrease the turnaround time for reporting antimicrobial susceptibility results from blood culture isolates.
Methods:
An evaluation was performed on the reliability of a method for antimicrobial susceptibility testing of positive blood culture isolates from briefly incubated solid medium cultures.
Results:
The agreement between the evaluated and standard methods was 99.3%. The major and minor error rates were 0.4% and 0.3%, respectively, and no very major errors were observed.
Conclusion:
The inoculation of briefly incubated solid medium cultures into antimicrobial susceptibility testing panels is an easy and reliable technique, and helps to decrease the turnaround time for reporting antimicrobial susceptibility results of positive blood cultures.
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