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Updated: Mar 25, 2026

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
Published on: May 22, 2018
The UniProtKB guide to the human proteome
Lionel Breuza1, Sylvain Poux2, Anne Estreicher2
1SIB Swiss Institute of Bioinformatics, Centre Medical Universitaire, 1 Rue Michel Servet, Geneva 4, 1211, Switzerland lionel.breuza@isb-sib.ch.
Researchers can access high-quality, comprehensive human proteome data through the UniProt Knowledgebase (UniProtKB). This resource combines expert manual curation and automated annotation for reliable gene and protein information.
Area of Science:
- Bioinformatics
- Proteomics
- Genomics
Background:
- High-throughput technologies enable routine whole genome and proteome analysis.
- Researchers require comprehensive, high-quality gene and protein datasets for their organisms of interest.
Purpose of the Study:
- To describe the content of the complete human proteome within the UniProt Knowledgebase (UniProtKB).
- To illustrate how UniProtKB captures and structures the complexity of the human proteome.
Main Methods:
- Utilizing the human proteome as a case study.
- Describing the UniProtKB/Swiss-Prot resource.
- Highlighting the integration of manual expert curation and expert-driven automatic annotation.
Main Results:
- UniProtKB provides a comprehensive, high-quality, and traceable resource for the human proteome.
- The database effectively captures and structures the complexity of proteomic data.
- Expert curation and automated annotation synergize to ensure data reliability.
Conclusions:
- UniProtKB is an essential resource for researchers performing whole genome and proteome analysis.
- The combination of manual and automated annotation ensures the quality and completeness of proteomic information.
- UniProtKB facilitates a deeper understanding of complex proteomes like the human proteome.
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