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Related Experiment Video

Updated: Mar 25, 2026

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

Antigen expression on a putative leukemic stem cell population and AML blast.

Swati Garg¹, Kanjaksha Ghosh¹, Manisha Madkaikar²

¹National Institute of Immunohaematology, 13th Floor, KEM Hospital Campus, Mumbai, 400012, India.

International Journal of Hematology

|February 25, 2016

Summary

This study investigated antigen expression differences between acute myeloid leukemia (AML) blasts and leukemic stem cells (LSCs). Researchers found no significant differences, suggesting AML blasts are suitable for antigen evaluation.

Keywords:

Acute myeloid leukemia CD44 CD96 Putative leukemic stem cells

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Hematology
Oncology
Immunology

Background:

A specific population of leukemic stem cells (LSCs) in acute myeloid leukemia (AML), defined as Lin-/CD38-/CD34+, impacts disease prognosis.
Differential antigen expression on normal versus leukemic stem cells is crucial for AML prognosis, yet has not been thoroughly studied on AML blasts and putative LSCs (pLSCs) in clinical settings.

Purpose of the Study:

To identify differences in the expression levels of CD44, CD90, CD96, CD123, CD25, and intracellular WT-1 between AML blasts and the Lin-/CD38-/CD34+ population in CD34+ AML patients.
To compare the expression intensity ratios of these markers on pLSCs versus bulk blast populations.

Main Methods:

Utilizing multi-parametric flow cytometry to analyze marker expression on AML blasts and the Lin-/CD38-/CD34+ population from 30 AML samples.
Performing paired analysis of marker expression intensity ratios between pLSC and blast populations.

Main Results:

No statistically significant differences were observed in the expression levels of the investigated markers (CD44, CD90, CD96, CD123, CD25, WT-1) between bulk AML blasts and the defined Lin-/CD38-/CD34+ subpopulation.
The expression intensity ratios of these antigens did not show significant variation between pLSC and blast populations.

Conclusions:

The study suggests that evaluating antigen expression on bulk AML blasts is sufficient and more practical than focusing on the pLSC population.
This finding has economic and informative implications for AML prognosis and potentially therapeutic targeting strategies.