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Rational design of hybridization chain reaction monomers for robust signal amplification
Yan Shan Ang1, Lin-Yue Lanry Yung1
1Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585. cheyly@nus.edu.sg.
Abstract:
We established four-point guidelines for the sequence design of hairpin monomers in hybridization chain reaction (HCR). This enabled greater flexibility to customize specific hairpin sequences for use with the readout platform of interest. Using shorter hairpin stem length, a one-pot signal amplification system was demonstrated by incorporating distance-sensitive Förster resonance energy transfer (FRET) readout.
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