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Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
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Growth factor release from dentine matrix by pulp-capping agents promotes pulp tissue repair-associated events
P L Tomson1, P J Lumley1, A J Smith1
1Oral Biology, The University of Birmingham College of Medical and Dental Sciences, School of Dentistry, Birmingham, UK.
International Endodontic Journal
|February 26, 2016
Summary
Mineral Trioxide Aggregate (MTA) and calcium hydroxide release growth factors from dentine. MTA significantly enhances pulp cell growth and migration, crucial for wound healing.
Area of Science:
- Dental Materials Science
- Biomaterials Engineering
- Cell Biology
Background:
- Pulp-capping agents aim to preserve dental pulp vitality.
- Understanding their interaction with dentine extracellular matrix (dECM) is crucial for optimizing regenerative potential.
Purpose of the Study:
- To characterize growth factor release from dentine by pulp-capping agents.
- To determine the effects of liberated dECM components on pulp cell migration and growth.
Main Methods:
- Dentine was exposed to calcium hydroxide and white/grey MTA for 14 days.
- Released dECM components were analyzed using ELISA and FTIR.
- Primary rat dental pulp cells (RDPCs) were assessed for growth and chemotaxis.
Main Results:
- Pulp-capping agents liberated diverse growth factors, with MTA releasing more than calcium hydroxide.
- MTA-released dECM components significantly enhanced RDPC proliferation and chemotaxis.
- FTIR revealed partial depletion of amide bands in dentine after agent exposure.
Conclusions:
- White and grey MTA effectively solubilize bioactive molecules from dentine.
- These molecules stimulate pulp cell proliferation and chemotaxis, promoting wound healing.
