Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Electron Transport Chain

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...

Anthelminthic Agents

Anthelminthic Agents

Anthelmintic drugs differ significantly from antiparasitic therapies targeting protozoa, primarily due to differences in parasite biology. Whereas most protozoal treatments act on proliferating cells, anthelmintics are typically directed against mature, nonproliferative helminths. The therapeutic approach considers the helminth's reliance on neuromuscular coordination, glucose metabolism, and microtubular integrity for survival, reproduction, and localization within the host. Most anthelmintics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cross-stage single-cell and spatial metabolome analyses reveal periderm specialization and tanshinone biosynthesis in Salvia miltiorrhiza roots.

The New phytologist·2026

Same author

Integrative multi-omics and radiomics reveal a TMSB10-driven cell state for non-invasive assessment and precision stratification in breast cancer.

Frontiers in immunology·2026

Same author

Correction: Berberine attenuates ischemia reperfusion injury through inhibiting HMGB1 release and NF-κB nuclear translocation.

Acta pharmacologica Sinica·2026

Same author

Extracellular vesicles in plants.

Molecular plant·2026

Same author

Gamma-selinene synthase catalyzes the first step of dihydroagarofuran sesquiterpene alkaloid biosynthesis in Tripterygium.

Science China. Life sciences·2025

Same author

A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges.

Nature communications·2025

Search research articles

Related Experiment Video

Updated: Mar 25, 2026

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Published on: March 7, 2025

Targeting insect mitochondrial complex I for plant protection.

Xiu-Ming Wu¹, Chang-Qing Yang¹, Ying-Bo Mao¹

¹National Key Laboratory of Plant Molecular Genetics and National Plant Gene Research Center (Shanghai), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Plant Biotechnology Journal

|February 26, 2016

Summary

Targeting the NDUFV2 gene in plants with double-stranded RNA (dsRNA) effectively controls insect pests. This mitochondrial complex I target shows high lethality and broad applicability for new insect-proof crops.

Keywords:

Helicoverpa armigera NDUFV2 RNA interference cotton mitochondrial complex I pest control

More Related Videos

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Published on: May 26, 2023

Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants

Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants

Published on: December 22, 2023

Related Experiment Videos

Last Updated: Mar 25, 2026

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Published on: March 7, 2025

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Author Spotlight: Development and Challenges of Feeding Assays for Insect Pest Management

Published on: May 26, 2023

Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants

Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants

Published on: December 22, 2023

Area of Science:

Agricultural Science
Molecular Biology
Entomology

Background:

RNA interference (RNAi) offers a novel pest control strategy.
Effective pest control requires potent gene targets for RNAi applications.
The mitochondrial complex I is crucial for insect respiration.

Purpose of the Study:

To identify and validate potent insecticidal targets for RNAi-based crop protection.
To evaluate the efficacy of targeting the NDUFV2 gene in insects.
To assess the potential of mitochondrial complex I as a broad-spectrum target.

Main Methods:

Engineering plants to express double-stranded RNA (dsRNA) targeting the NDUFV2 gene.
Feeding studies with cotton bollworm (Helicoverpa armigera) larvae using transgenic cotton.
Assessing insect mortality, mitochondrial structure and activity, and gene expression.
Reciprocal assays with Asian corn borer (Ostrinia furnacalis) and testing on Apolygus lucorum.

Main Results:

Suppression of NDUFV2 dsRNA in cotton caused up to 80% mortality in Helicoverpa armigera larvae within 5 days.
Altered mitochondrial structure and activity were observed in affected insects.
Transcriptome analysis revealed significant repression of dopa decarboxylase genes.
NDUFV2 suppression demonstrated sequence specificity and affected multiple insect species, including Ostrinia furnacalis and Apolygus lucorum.

Conclusions:

The NDUFV2 gene, a subunit of mitochondrial complex I, is a highly effective and lethal target for RNAi-based insect pest control.
Mitochondrial complex I represents a promising target for developing next-generation insect-proof crops due to its sequence specificity and wide applicability across different insect orders.