High-Throughput Measurement of Binding Kinetics by mRNA Display and Next-Generation Sequencing
Farzad Jalali-Yazdi1, Lan Huong Lai2, Terry T Takahashi2
1Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA, 90089, USA.
Angewandte Chemie (International Ed. in English)
|February 26, 2016
Abstract:
There is great demand for high-throughput methods to characterize ligand affinity. By combining mRNA display with next-generation sequencing, we determined the kinetic on- and off-rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement.
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