Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

24.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
MicroRNAs01:22

MicroRNAs

12.0K
12.0K
Experimental RNAi02:15

Experimental RNAi

8.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.2K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.0K
RNA Interference01:23

RNA Interference

28.6K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-Dimensional Architectures for Silicon Wafer-Based Integrated Microenergy Storage Systems.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Bright nanoparticle-labeled lateral flow immunoassay for rapid quantitative detection of tacrolimus in human plasma.

Mikrochimica acta·2025
Same author

Comparison of Predictive Performance of Three Lymph Node Staging Systems in Colorectal Signet Ring Cell Carcinoma Based on Machine Learning Model.

Journal of visualized experiments : JoVE·2025
Same author

An interpretable machine learning model based on optimal feature selection for identifying CT abnormalities in patients with mild traumatic brain injury.

EClinicalMedicine·2025
Same author

Metabolomic machine learning-based model predicts efficacy of chemoimmunotherapy for advanced lung squamous cell carcinoma.

Frontiers in immunology·2025
Same author

Serum Albumin Levels and Length of Stay in Premature Infants.

Indian journal of pediatrics·2025

Related Experiment Video

Updated: Mar 25, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

8.0K

[Research progress in the function of microRNA-182].

Mei-Juan Dong1,2, Ting Xiao1, Wen Meng1

  • 1Metabolic Syndrome Research Center of Central South University, the Second Xiangya Hospital of Central South University, Key Laboratory of Diabetes Immunology, Ministry of Education, Central South University, Changsha 410011, China.

Sheng Li Xue Bao : [Acta Physiologica Sinica]
|February 27, 2016
PubMed
Summary

MicroRNA-182 (miR-182) plays key roles in cell functions and is implicated in diseases like cancer and diabetes. Understanding miR-182

More Related Videos

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

37.0K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K

Related Experiment Videos

Last Updated: Mar 25, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

8.0K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

37.0K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • microRNAs (miRNAs) are short non-coding RNAs regulating gene expression.
  • miRNA-182 (miR-182) is part of the miR-183/96/182 cluster and highly expressed in various tissues.
  • miR-182 is crucial for cellular differentiation, development, and tissue maintenance.

Purpose of the Study:

  • To review the roles and mechanisms of miR-182 in cellular functions.
  • To summarize the involvement of miR-182 in various diseases.
  • To highlight recent research progress on miR-182.

Main Methods:

  • Literature review of recent studies on miR-182.
  • Analysis of miR-182's function in cellular processes.
  • Examination of miR-182's association with disease development.

Main Results:

  • miR-182 is involved in cellular differentiation, proliferation, apoptosis, and metabolism.
  • Dysregulation of miR-182 is linked to retinopathy, autoimmune diseases, cancers, obesity, and diabetes.
  • miR-182 plays significant roles in the functional maintenance of various cells and tissues.

Conclusions:

  • miR-182 is a critical regulator of cellular functions.
  • Aberrant miR-182 expression contributes to the pathogenesis of numerous diseases.
  • Further research into miR-182 mechanisms can offer therapeutic insights.