Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

2.0K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
2.0K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

2.1K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
2.1K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

1.2K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
1.2K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

840
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
840

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodality staging of renal cell carcinoma-pearls & pitfalls.

Abdominal radiology (New York)·2026
Same author

Reconstruction of the Achilles tendon using LARS artificial ligament in bilateral xanthoma: a case report and literature review.

BMC surgery·2026
Same author

Imaging the hallmarks of cancer.

Nature reviews. Cancer·2026
Same author

Affinity-Tuned Albumin Hitchhiking Extends the Bioorthogonal Capture Window in Pretargeting Radiotheranostics.

Advanced healthcare materials·2026
Same author

The effect of active warming and conventional body temperature management on perioperative blood loss and coagulation function in patients undergoing scoliosis correction: a multicenter randomized controlled trial.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Potential Clinical Applications of Hyperpolarized <sup>13</sup>C and <sup>2</sup>H MRI.

Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer·2026

Related Experiment Video

Updated: Mar 25, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.1K

Single shot three-dimensional pulse sequence for hyperpolarized 13 C MRI.

Jiazheng Wang1, Alan J Wright1, De-En Hu1

  • 1Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge, United Kingdom.

Magnetic Resonance in Medicine
|February 27, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a new 3D imaging sequence for hyperpolarized 13C metabolic imaging. The technique enables faster, more robust imaging of [1-13C]pyruvate metabolism in tumors, improving in vivo metabolic insights.

Keywords:
imaginglactatemetabolismpyruvatespiral trajectorytumors

More Related Videos

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.2K
Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

1.6K

Related Experiment Videos

Last Updated: Mar 25, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.1K
Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.2K
Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Published on: July 8, 2025

1.6K

Area of Science:

  • Medical Imaging
  • Metabolic Imaging
  • Magnetic Resonance Imaging

Background:

  • Metabolic imaging using hyperpolarized 13C substrates offers in vivo tissue metabolism insights.
  • The short lifetime of hyperpolarization limits imaging time and excitation pulses.

Purpose of the Study:

  • To develop a single-shot 3D imaging sequence for hyperpolarized 13C metabolic imaging.
  • To demonstrate its capability in generating 13C MR images of tumors in vivo.

Main Methods:

  • A novel pulse sequence was designed, acquiring data using a stack-of-spirals at two spin echoes after a single excitation.
  • Spectral-spatial pulses were employed for dynamic 3D imaging of hyperpolarized 13C metabolites.
  • The sequence was tested in tumor-bearing mice using hyperpolarized [1-13C]pyruvate.

Main Results:

  • Achieved nominal spatial/temporal resolution of 1.25 × 1.25 × 2.5 mm3 × 2 s for in vivo tumor imaging.
  • Successfully acquired dynamic 3D images of hyperpolarized [1-13C]pyruvate and [1-13C]lactate.
  • Demonstrated higher z-direction resolution in phantom studies with [1-13C]lactate.

Conclusions:

  • The developed pulse sequence enables high-resolution, robust 3D imaging of hyperpolarized 13C substrates.
  • This technique overcomes limitations of hyperpolarization decay for improved metabolic imaging.
  • The method shows promise for in vivo assessment of tissue metabolism.