Protein crystallography beamline (PX-BL21) at Indus-2 synchrotron

Area of Science:

• Structural Biology
• Biophysics
• Materials Science

Background:

• The Indian synchrotron (Indus-2) now hosts a dedicated protein crystallography beamline, PX-BL21.
• This beamline is situated at a 1.5 T bending-magnet port, enhancing research capabilities.

Purpose of the Study:

• To announce the availability and describe the capabilities of the new protein crystallography beamline (PX-BL21).
• To detail the instrumentation and user facilities for macromolecular X-ray diffraction studies.

Main Methods:

• Utilizes X-ray diffraction for single-crystal analysis of macromolecules.
• Operates within a 5-20 keV energy range for accessing heavy element absorption edges.
• Employs a double-crystal monochromator and X-ray mirrors for beam control.

Main Results:

• The beamline is equipped with a goniometer, CCD detector, fluorescence detector, cryo-cooler, and automated sample changer.
• On-site data processing and sample preparation facilities are available to users.
• Initial scientific results demonstrate the beamline's functionality.

Conclusions:

• PX-BL21 at Indus-2 is a valuable new resource for macromolecular crystallography research in India.
• The beamline's comprehensive facilities support efficient structural determination of proteins and nucleic acids.