Enzymatic approaches for studying the structure, synthesis, and processing of glycoproteins

The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.

The Journal of biological chemistry·2001

Structural basis for the substrate specificity of endo-beta-N-acetylglucosaminidase F(3).

Biochemistry·2000

The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing.

The Journal of biological chemistry·2000

Selective organic precipitation/extraction of released N-glycans following large-scale enzymatic deglycosylation of glycoproteins.

Analytical biochemistry·2000

Two-dimensional relayed-rotating-frame overhauser spectroscopy (1)H NMR experiments for the selective identification of 1,2-glycosidic linkages in polysaccharides.

Analytical biochemistry·2000

Novel Schizosaccharomyces pombe N-linked GalMan9GlcNAc isomers: role of the Golgi GMA12 galactosyltransferase in core glycan galactosylation.

Glycobiology·1999

Ferroptosis induction via genetic approaches - CRISPR/Cas9-based disruption on key anti-ferroptotic genes.

Methods in cell biology·2026

Optimized strategies for necroptosis induction and evaluation.

Methods in cell biology·2026

In vitro culture of intestinal organoids for cell death assessments.

Methods in cell biology·2026

Assessing the activity of caspase-activated pannexin-1 channels by flow cytometry.

Methods in cell biology·2026

Sensitive detection of lysosomal membrane permeabilization using the galectin puncta assay.

Methods in cell biology·2026

High-throughput screening for cell death inducers and inhibitors.

Methods in cell biology·2026

Identification of Senescence-Associated β-Galactosidase With Single-Molecule Resolution.

Angewandte Chemie (International ed. in English)·2026

Sphingolipid Metabolism in Obesity: Bidirectional Regulation and Comparative Perspectives on Plant Sphingolipids.

Nutrients·2026

Brain O-GlcNAcylation in Neurodegenerative Diseases: Context-Dependent Mechanisms and Precision Therapeutic Translation.

Brain sciences·2026

Serum Asprosin Levels in Patients with Hidradenitis Suppurativa: A Case-Control Study.

Diagnostics (Basel, Switzerland)·2026

Impacts of N-glycanase1 (NGLY1) Down Regulation on the Function of Mitochondria.

Journal of cellular biochemistry·2026

Bidirectional Allosteric Ligand Regulation in a Central Glycolytic Enzyme.

Journal of the American Chemical Society·2026

Related Experiment Videos

Enzymatic approaches for studying the structure, synthesis, and processing of glycoproteins

Related Concept Videos

The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.

Structural basis for the substrate specificity of endo-beta-N-acetylglucosaminidase F(3).

The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing.

Selective organic precipitation/extraction of released N-glycans following large-scale enzymatic deglycosylation of glycoproteins.

Two-dimensional relayed-rotating-frame overhauser spectroscopy (1)H NMR experiments for the selective identification of 1,2-glycosidic linkages in polysaccharides.

Novel Schizosaccharomyces pombe N-linked GalMan9GlcNAc isomers: role of the Golgi GMA12 galactosyltransferase in core glycan galactosylation.

Ferroptosis induction via genetic approaches - CRISPR/Cas9-based disruption on key anti-ferroptotic genes.

Optimized strategies for necroptosis induction and evaluation.

In vitro culture of intestinal organoids for cell death assessments.

Assessing the activity of caspase-activated pannexin-1 channels by flow cytometry.

Sensitive detection of lysosomal membrane permeabilization using the galectin puncta assay.

High-throughput screening for cell death inducers and inhibitors.

Identification of Senescence-Associated β-Galactosidase With Single-Molecule Resolution.

Sphingolipid Metabolism in Obesity: Bidirectional Regulation and Comparative Perspectives on Plant Sphingolipids.

Brain O-GlcNAcylation in Neurodegenerative Diseases: Context-Dependent Mechanisms and Precision Therapeutic Translation.

Serum Asprosin Levels in Patients with Hidradenitis Suppurativa: A Case-Control Study.

Impacts of N-glycanase1 (NGLY1) Down Regulation on the Function of Mitochondria.

Bidirectional Allosteric Ligand Regulation in a Central Glycolytic Enzyme.

Related Experiment Videos

Enzymatic approaches for studying the structure, synthesis, and processing of glycoproteins

Related Concept Videos

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