Related Experiment Videos
Enzyme catalyzed acylation and deacylation of the sugar moieties of nucleosides
K Nozaki1, A Uemura, J Yamashita
1Chemical Synthesis Research Laboratory, TAIHO Pharmaceutical Co., Ltd., Saitama, Japan.
Nucleic Acids Symposium Series
|January 1, 1989
Abstract:
We have found that a lipase from Pseudomonas fluorescens (PFL) accelerated regioselective acylation of 2'-deoxynucleosides with the use of acid anhydrides as acyl donor in dry polar solvents. Different regioselective deacylation of 3',5'-di-O-acyl-2'-deoxynucleosides was found to take place when a lipase (PFL) or a protease from Bacillus subtilis (Subtilisin) was used.