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Published on: March 24, 2017
Recognition of a multiple antigen peptide containing sequence from mimotope of the dengue type 3 virus NS4B protein
Nevis Amin1, Maritza Pupo2, Alicia Aguilar1
1Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute, 11600, Havana, Cuba.
Objective:
To evaluate the recognition of NS4B mimotope, as multiple antigen peptide (MAP), by dengue antibodies presents in serum samples from patients with different serotype infections.
Methods:
A MAP containing mimotope sequence was synthesized and used to evaluate the recognition of NS4B mimotope as MAP by a panel of 66 human sera from dengue cases by an indirect ELISA assay.
Results:
The MAP differentiated between sera from dengue viruses infected patients and sera from healthy individuals and the best reactivity was shown by serum from dengue type 3 virus patients. The recognition was more intense with serum from patients with secondary infection.
Conclusions:
The findings suggest the potential use of NS4B mimotope on the development of a multi-epitope diagnostic tool. These results are important for further immunogenicity studies.
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