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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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Related Experiment Video

Updated: Mar 25, 2026

Purification of Transcripts and Metabolites from Drosophila Heads
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Strand-specific RNA-sequencing analysis of multiple system atrophy brain transcriptome.

J D Mills1, M Ward1, W S Kim2

  • 1School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.

Neuroscience
|March 1, 2016
PubMed
Summary
This summary is machine-generated.

Multiple system atrophy (MSA) disrupts long intervening non-coding RNAs (lincRNAs) and protein-coding genes in the brain. This study reveals novel lincRNAs and transcriptional changes contributing to MSA pathology.

Keywords:
antisense RNAhuman brainlong intervening non-coding RNAsmultiple system atrophystrand-specific RNA-Seq

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Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
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Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Multiple system atrophy (MSA) is a sporadic neurodegenerative disease characterized by alpha-synuclein accumulation in oligodendrocytes.
  • Unlike Parkinson's disease, MSA lacks a definitive familial etiology, but transcriptional dysregulation is increasingly implicated.

Purpose of the Study:

  • To investigate the transcriptional landscape of the MSA brain frontal cortex using RNA-sequencing.
  • To identify differentially expressed genes, particularly long intervening non-coding RNAs (lincRNAs), and their role in MSA pathology.

Main Methods:

  • Ribosomal-depleted strand-specific RNA-sequencing of MSA brain frontal cortex tissue.
  • Differential gene expression analysis and identification of lincRNAs.
  • Analysis of alternative splicing and antisense transcription.

Main Results:

  • Over 50% of 123 differentially expressed genes were identified as putative lincRNAs.
  • Dysregulation of protein-coding genes involved in iron metabolism (e.g., SERPINA3, HBB) and immune response (e.g., IL1RL1) was observed.
  • Alternative splicing of SNCA and widespread, largely unaffected, antisense transcription were noted.

Conclusions:

  • MSA significantly disrupts lincRNAs and protein-coding genes in the human brain.
  • Novel lincRNAs specific to MSA were discovered, adding complexity to its transcriptional pathology.
  • Findings highlight the role of transcriptional dysregulation, including lincRNAs, in MSA pathogenesis.