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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
Published on: February 26, 2018
Development of a disaggregation-induced emission probe for the detection of RecA inteins from Mycobacterium
Xin Li1, Hao Jiang2, Zhaofeng Luo2
1Institute of Technical Biology and Agriculture Engineering, Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China. zly0605@ustc.edu.cn.
Abstract:
A fluorescent sensor - InR - with disaggregation-induced emission (DIE) characteristics has been developed for the label free detection of RecA inteins from Mycobacterium tuberculosis. Mechanistic studies demonstrate that InR binds at a hydrophobic pocket of RecA inteins, which is constituted by Gly435, Glu434, Arg405 and Arg 7, resulting in the disaggregation and recovery of its fluorescence.
