3', 4'-dihydroxyflavonol attenuates tissue damage in unilateral testis ischemia-reperfusion in rats

The purpose of this study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on oxidative damage and antioxidant system in experimental testicular torsion-detorsion.The study included 60 male Wistar albino rats. Study groups were formed as follows: 1. Control; 2. Sham; 3. 720° - 4 hours torsion; 4. 720° - 4 hours torsion + 4 hours detorsion; 5. 720° - 4 hours torsion + DiOHF; 6. 720° - 4 hours torsion + DiOHF + 4 hours detorsion; 7. 720° - 4 hours torsion + 24 hours detorsion; 8. 720° - 4 hours torsion + DiOHF + 24 hours detorsion. Testis were collected for the analysis of glutathione peroxidase (GPx), nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), and xanthine oxidase (XO).GPx in the Group 8 were higher than the values in the other groups (p < 0.001). Concerning NO, the groups 3, 4, and 7 were found to have higher values than other groups (p < 0.001). MDA levels were higher in the groups 3, 7, and 8, when compared to the levels in other groups (p < 0.001). When tissue GSH levels were examined, the Group 5 had the highest GSH values (p < 0.001).With regard to XO values, the groups 3, 4, and 7 had the highest XO values (p < 0.001). The results of the study indicated that intraperitoneal DiOHF inhibited increased lipid peroxidation in testis ischemia-reperfusion injury in rats (Tab. 5, Ref. 46).