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Related Experiment Videos

Anaplasma marginale in tick cell culture.

R J Hidalgo1, E W Jones, J E Brown

  • 1College of Veterinary Medicine, Mississippi State University 39762.

American Journal of Veterinary Research
|December 1, 1989
PubMed
Summary

Anaplasma marginale was successfully cultured in a tick cell line from Dermacentor variabilis embryos. This research establishes a continuous Anaplasma marginale culture for further study.

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Area of Science:

  • Veterinary Entomology
  • Microbiology
  • Cell Biology

Background:

  • Anaplasma marginale is an economically significant tick-borne pathogen.
  • Establishing continuous cell cultures is crucial for studying tick-borne rickettsial organisms.

Purpose of the Study:

  • To propagate Anaplasma marginale in a tick cell line.
  • To characterize the growth and morphology of Anaplasma marginale in vitro.

Main Methods:

  • Propagation of Anaplasma marginale in a Dermacentor variabilis embryonic cell line.
  • Monitoring using transmission electron microscopy and indirect immunofluorescence with monoclonal antibodies.
  • Continuous culture maintenance through multiple passages over extended periods.

Main Results:

  • Three continuous Anaplasma marginale cultures were established from infected tick tissues.
  • Organisms initiated growth near the nuclear membrane and formed cytoplasmic inclusions.
  • Maximal growth occurred between 7-14 days, with new growth cycles initiated upon cell transfer.
  • Morphology varied from erythrocyte-associated forms to midgut-like forms after several passages.

Conclusions:

  • A tick cell line derived from Dermacentor variabilis embryos supports continuous Anaplasma marginale propagation.
  • The established cell culture system provides a valuable tool for studying Anaplasma marginale.
  • In vitro culture allows for observation of Anaplasma marginale's life cycle and morphological changes.

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