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Updated: Aug 7, 2026
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
Identification of a gene encoding a putative phosphotransferase system enzyme IIBC in Listeria welshimeri and its
D Liu1, A J Ainsworth, F W Austin
1College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.
Aims:
To identify a Listeria welshimeri-specific gene that can be used for identification of this species by PCR.
Methods And Results:
Through comparative analysis of genomic DNA from Listeria species using dot blot hybridization, an L. welshimeri-specific clone was isolated that contained a gene segment whose translated protein sequence is similar to enzyme IIBC from phosphotransferase systems in other bacteria. Using oligonucleotide primers derived from this L. welshimeri-specific clone, a 608-bp fragment was amplified from L. welshimeri genomic DNA and not from other Listeria species or other Gram-negative and Gram-positive species.
Conclusion And Significance:
The PCR employing L. welshimeri-specific primers shows promise as a useful method for differentiating L. welshimeri from other Listeria species and related bacteria.
