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Related Experiment Video

Updated: Mar 24, 2026

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

Spatially-resolved protein surface microsampling from tissue sections using liquid extraction surface analysis.

Maxence Wisztorski¹, Annie Desmons¹, Jusal Quanico¹

¹Univ. Lille, Inserm, U1192 - Protéomique Réponse Inflammatoire Spectrométrie de Masse - PRISM, Lille, France.

|March 2, 2016

Summary

This summary is machine-generated.

This study introduces a novel liquid-microjunction extraction method for rapid, reproducible tissue proteomics. The technique allows precise mapping of the tissue microenvironment at millimeter resolution, aiding disease pathology understanding.

Keywords:

LESA Protein micro-extraction Surface sampling Technology Tissue microproteomics

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Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

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Published on: January 13, 2023

Area of Science:

Proteomics
Molecular Biology
Biotechnology

Background:

Tissue microenvironment characterization is crucial for understanding complex pathologies.
Precise disease phenotyping requires efficient microsampling and accurate localization for region-dependent proteomics.

Purpose of the Study:

To develop a method for rapid and reproducible protein extraction from specific tissue regions at millimeter scale.
To enable region-dependent proteomics for detailed pathology analysis.

Main Methods:

Liquid-microjunction extraction using conventional detergent solution.
Analysis of proteins from a 1-mm diameter spot size on tissue sections.
Immunoblotting experiments for protein retrieval and identification.

Main Results:

Successfully retrieved and identified over 1400 proteins from 1-mm tissue spots with high reproducibility.
Demonstrated qualitative and quantitative reproducibility in protein analysis.
Successfully mapped distinct brain regions on rat sagittal sections by analyzing proteins from ten different points.

Conclusions:

The developed method enables rapid, reproducible, and region-specific protein extraction from tissue sections.
This technology facilitates functional mapping and understanding of molecular mechanisms within the tissue microenvironment at millimeter resolution.
The approach significantly advances the characterization of disease pathology through precise spatial proteomics.