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A surface acoustic wave (SAW)-enhanced grating-coupling phase-interrogation surface plasmon resonance (SPR)
A Sonato1, M Agostini2, G Ruffato3
1CNR-IOM, Area Science Park, S.S. 14, km 163.5, 34149, Basovizza (TS), Italy.
Lab on a Chip
|March 3, 2016
Summary
This study introduces a novel microfluidic biosensor combining surface acoustic wave (SAW) mixing with surface plasmon resonance (SPR) for enhanced molecule binding. The device significantly speeds up binding kinetics, offering a portable solution for diverse biosensing applications.
Area of Science:
- * Nanotechnology and Microfluidics
- * Biosensing and Surface Chemistry
- * Acoustic and Optical Physics
Background:
- * Traditional biosensors face limitations in sensitivity and speed.
- * Microfluidic devices offer miniaturization but often lack efficient mixing.
- * Surface plasmon resonance (SPR) and surface acoustic wave (SAW) technologies have independent applications in sensing.
Purpose of the Study:
- * To develop a novel microfluidic biosensor integrating SAW and SPR.
- * To enhance molecule binding kinetics through SAW-induced chaotic advection.
- * To demonstrate the device's utility for real-time kinetic monitoring.
Main Methods:
- * Fabrication of a lab-on-a-chip device using lithium niobate.
- * Integration of phase-interrogation grating-coupling SPR with SAW.
- * Monitoring of thiol-polyethylene glycol and avidin/biotin binding kinetics.
Main Results:
- * Achieved significant reduction in binding time: 82% for polyethylene and 24% for avidin.
- * Demonstrated enhanced fluid mixing via SAW-generated chaotic advection.
- * Validated the high sensitivity of grating-coupling SPR under azimuthal control.
Conclusions:
- * The developed SAW-SPR microfluidic biochip is the first of its kind.
- * The device significantly improves molecule binding kinetics on a portable platform.
- * The proposed biochip is versatile for various biosensing applications.
