Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Techniques for Isolation of Pure Cultures

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Precipitation and Co-precipitation

Precipitation and Co-precipitation

Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...

Extraction: Advanced Methods

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same journal

Molecular Docking of Bioactive Compounds Against BRCA and COX Proteins.

Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques·2016

Same journal

Detection of Phenolic and Flavonoid Compounds Using High Performance Thin Layer Chromatography (HPTLC).

Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques·2016

Same journal

Protein Bioavailability in Animal Model.

Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques·2016

Same journal

Anti-tumour Activity.

Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques·2016

Same journal

Induction of Apoptosis.

Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques·2016

Same journal

Determination of Cytotoxicity.

Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques·2016

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 24, 2026

Isolating Free Carbenes, their Mixed Dimers and Organic Radicals

Isolating Free Carbenes, their Mixed Dimers and Organic Radicals

Published on: April 19, 2019

Isolation of Compounds

Progress in Drug Research. Fortschritte Der Arzneimittelforschung. Progres Des Recherches Pharmaceutiques

|March 5, 2016

Summary

This summary is machine-generated.

This study details methods for isolating plant compounds, crucial for understanding their therapeutic effects. Techniques like thin-layer chromatography (TLC) and column chromatography are explained for accurate identification.

More Related Videos

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol

Published on: April 2, 2018

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

Published on: February 7, 2019

Related Experiment Videos

Last Updated: Mar 24, 2026

Isolating Free Carbenes, their Mixed Dimers and Organic Radicals

Isolating Free Carbenes, their Mixed Dimers and Organic Radicals

Published on: April 19, 2019

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol

Published on: April 2, 2018

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

Published on: February 7, 2019

Area of Science:

Phytochemistry
Pharmacognosy
Analytical Chemistry

Background:

Plants are rich sources of bioactive chemical compounds.
Understanding these compounds is key to unlocking plant-based therapeutic potential.
Accurate identification of plant-derived compounds is essential for medicinal applications.

Purpose of the Study:

To describe detailed methods for the isolation of chemical compounds from plants.
To highlight the importance of precise compound identification in pharmacognosy.
To provide a guide for researchers screening plant-derived bioactive molecules.

Main Methods:

Detailed explanation of isolation techniques.
Focus on thin-layer chromatography (TLC) for initial separation and analysis.
Description of column chromatography for purification of isolated compounds.

Main Results:

A clear protocol for isolating plant chemical constituents is presented.
The study emphasizes the utility of TLC and column chromatography in this process.
Methodology ensures careful handling and attention for reliable results.

Conclusions:

Effective isolation of plant compounds is achievable using described chromatographic methods.
This work aids in the identification of bioactive molecules for therapeutic research.
Precise isolation techniques are fundamental to advancing phytomedicine.