Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.3K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.3K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.5K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.5K
CRISPR01:59

CRISPR

58.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MicroRNA-195-5p/E2F7 Axis Underlies the Antimicrobial Additive Triclosan-Induced Cytotoxicity in Human Pulmonary Epithelial Cells.

Journal of applied toxicology : JAT·2026
Same author

Shifting paradigms from myeloablation to immune modulation: pre-transplant immune-suppression and post-transplant cyclophosphamide in human leucocyte antigen identical related donor hematopoietic stem cell transplantation for sickle cell disease.

Haematologica·2026
Same author

Mutations in KAT3 family of lysine acetyl transferases impair neural crest migration in Rubinstein Taybi syndrome models.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same author

AI-guided CAR designs and targeted pathway modulation to enhance multi-antigen CAR T cell durability and overcome antigen escape.

Nature communications·2026
Same author

CRISPR editing of HPFH3 genotype induces γ-globin expression and reverses sickle cell disease and β-thalassemia phenotypes.

Stem cell research & therapy·2025
Same author

Decoding the multifaceted role of erythrocyte PMCA4b in oxidative stress-mediated malaria protection and artemisinin resistance.

mBio·2025

Related Experiment Video

Updated: Mar 24, 2026

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.3K

Programmable Site-Specific Nucleases for Targeted Genome Engineering in Higher Eukaryotes.

Ganesan Govindan1, Sivaprakash Ramalingam1

  • 1Department of Biotechnology, Molecular Biology Laboratory, MS Swaminathan Research Foundation, Taramani, Chennai, India.

Journal of Cellular Physiology
|March 7, 2016
PubMed
Summary
This summary is machine-generated.

Targeted genome engineering uses site-specific nucleases like ZFNs, TALENs, and CRISPR-Cas9 to precisely edit DNA. These tools enable gene knockouts or specific modifications for disease treatment and agricultural applications.

More Related Videos

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

29.4K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K

Related Experiment Videos

Last Updated: Mar 24, 2026

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.3K
Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

29.4K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Advancements in targeted genome engineering allow precise DNA modifications in eukaryotic genomes.
  • Programmable site-specific DNA cleavage reagents and cellular repair pathways are key to these modifications.

Purpose of the Study:

  • To summarize the role of zinc finger nucleases (ZFNs), transcription activator like effector nucleases (TALENs), and CRISPR-Cas9 in targeted genome modification.
  • To discuss the applications of programmable site-specific nucleases (SSNs) in human disease treatment and in higher eukaryotic organisms.

Main Methods:

  • Utilizing site-specific nucleases to create targeted genomic double-strand breaks (DSBs).
  • Employing non-homologous end joining (NHEJ) for gene inactivation or homology-directed repair (HDR) for sequence modification using donor DNA templates.

Main Results:

  • ZFNs, TALENs, and CRISPR-Cas9 systems are effective tools for targeted genome engineering.
  • These nucleases facilitate both gene knockouts and precise sequence alterations at desired genomic loci.

Conclusions:

  • Programmable site-specific nucleases offer powerful capabilities for genetic manipulation.
  • Applications are expanding into human therapeutics and improvements in plants and livestock.